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Description
Alpaca anti-SNAP/CLIP-tag VHH, purified recombinant binding protein
Alpaca anti-SNAP/CLIP-tag VHH, purified recombinant binding protein
v1,1/5 mm/pagealloc: convert 'report' flag of freeonepage to a proper flag mm: place pages to the freelist tail when onling and undoing isolation 1 5 - 0 0 0: 2020-09-28: David Hildenbrand: New RFC,v2,30/30 mm: thp: enable anonymous PUD THP at page fault path. 1GB PUD THP support on x8664 -0 0 0: 2020-09-28: Zi Yan: New. Using 32 mm (1.26 in) - 0 gauge - track, there is an extensive range of 16 mm to the foot scale 1:19 live-steam and other types of locomotives, rolling stock and accessories. Many of these models are dual gauge, and can be converted to run on 45 mm ( 1.772 in ) track ( gauge 1 ), and radio control is common.
Synonyms
SNAP/CLIP-tag®-Binding-Protein, SNAP/CLIP-tag®-Nanobody, and SNAP/CLIP-tag®-single domain antibody (sdAb)
SNAP/CLIP-tag®-Binding-Protein, SNAP/CLIP-tag®-Nanobody, and SNAP/CLIP-tag®-single domain antibody (sdAb)
Specificity
SNAP-tag and CLIP-tag
SNAP-tag and CLIP-tag
Applications
Coupling of dyes, biotin, etc. via NHS Ester reaction
Coupling of dyes, biotin, etc. via NHS Ester reaction
Product | Size | Code | Price | Buy |
---|---|---|---|---|
ProductSNAP/CLIP-tag® VHH, recombinant binding protein | Size250 µL | Codewt-250 | Price |
Affinity
Dissociation constant KD of 1 nM
Dissociation constant KD of 1 nM
Specificity
SNAP-tag & CLIP-tag
SNAP-tag & CLIP-tag
Conjugation:
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
Unconjugated
Suitable for conjugation of dyes, biotin, beads, surfaces etc. via NHS ester reaction
5 1 Tall
Biophysical properties
Molecular weight MW: 13.1 kDa
Molecular extinction coefficient ε: 24,075 L·Mol−1·cm−1
Isoelectrical point pI: 9.8 (theoretical)
Molecular weight MW: 13.1 kDa
Molecular extinction coefficient ε: 24,075 L·Mol−1·cm−1
Isoelectrical point pI: 9.8 (theoretical)
Form
Purified recombinant antibody fragment, monoclonal Outline 3 21 3.
Purified recombinant antibody fragment, monoclonal Outline 3 21 3.
Protein concentration
1 mg/mL
1 mg/mL
Storage buffer
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09% sodium azide
25mM TAPS pH 8.5, 500 mM NaCl, 5 mM EDTA, preservative: 0.09% sodium azide
How to cite this product
RRID: AB_2827564
RRID: AB_2827564
Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one year.
Do not freeze.
What are the biophysical parameters of the Nanobodies?
Name | MW (kDa) | Molar Extinction Coefficient (M-1 cm-1) | Theoretical pI |
---|---|---|---|
GFP VHH | 13.9 | 27,055 | 9.4 |
GST VHH | 14.8 | 28,545 | 8.2 |
Halo VHH | 14.8 | 23,045 | 9.4 |
Histon VHH | 14.4 | 25,565 | 10.2 |
MBP VHH | 14.1 | 27,055 | 10.0 |
Mdm4/Hdmx VHH | 13.6 | 14,565 | 10.1 |
MK2 VHH | 14.4 | 32,555 | 9.7 |
mNeonGreen VHH | 14.3 | 21,680 | 8.0 |
Myc VHH | 13.5 | 23,045 | 9.6 |
p53 C-term VHH | 14.8 | 34,170 | 9.2 |
p53 N-term VHH | 13.0 | 20,065 | 9.7 |
PARP1 VHH | 14.4 | 17,670 | 9.2 |
RFP VHH | 14.9 | 30,035 | 9.3 |
SNAP/CLIP-tag VHH | 13.1 | 24,075 | 9.8 |
Spot VHH (bivalent) | 30.3 | 46,340 | 5.3 |
TurboGFP VHH | 13.6 | 18,575 | 9.5 |
V5 VHH | 14.6 | 22,710 | 8.0 |
Vimentin VHH | 26.7 | 48,150 | 10.0 |
- Dissociation constant KD of 1 nM
- Compatible to the following wash conditions: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
- Applications validated and characterized
Tagr 5 1 0 Mm Equals How Many Inches
Only for research applications, not for diagnostic or therapeutic use!
5 1 Height Cm
KnittingNeedle Sizes
This chart lists the mostcommon metric equivalents for U.S.,'Old U.S.' and 'Old U.K' needle sizes. Different needlemanufacturers may vary from the exact sizes shown; however, ranges ofcontemporaryU.S. needles are generally as shown in the 'Contemporary U.K./U.S.Metric Range' column.
'Old U.S.' sizes are frequentlyseen in old yarncompany leaflets; since the sizes run opposite of contemporary sizing(underthe 'Old U.S.' system, the finest needles have the highest sizenumbers) the giveaway is a sock pattern (or other pattern usingfingering-weightyarn) that calls for size 13 needles! Note that the metric measurementsof U.K.sizes differ, so it may be important to know the source of yourpattern.
Source for the 'Old U.S.'column is a needle gaugefrom Boye Needle Company that is reproduced in CompleteGuide to ModernKnitting and Crocheting by AliceCarroll, published 1942 by Wise &Co. Source for all other information below is 'My Valuable Yarn Guide'(no author or publisher info appears on this publication).
This chart is lengthy; you mayneed to scroll down severaltimes to find the size you are looking for. To make navigation easier,wait until this page has finished loading. Then these links willquickly take you down to specific points on the chart:
Chart beginswith .5 mm (U.S. size 8/0) | Goto 2.0 mm (U.S. size 0) | Goto 4.5 mm (U.S. size 7) | Goto 8.0 mm (U.S. size 11) |
Contemporary U.S. Size | Contemporary U.K./U.S. Metric Range | EuropeanMetric Range | 'Old'U.S. (Taken from a Boye Needle gauge reprinted in a 1942 publication) | U.K. |
8/0 | .5mm | .5mm | N/A | 24 |
6/0 | .75mm | .75mm | N/A | 22 |
N/A | N/A | .875mm (?) | N/A | 20 |
5/0 | 1.0mm | 1.0mm | 18 (steeldpn) | 19 |
N/A | N/A | 1.125mm | 17 (steeldpn) | N/A |
4/0 | 1.25mm | 1.25mm | 16 (steeldpn) | 18 |
000 | 1.5mm | 1.5mm | 15 (steeldpn) | 17 |
N/A | N/A | 1.625mm (?) | N/A | 16 |
00 | 1.75mm | 1.75mm | 14 (steeldpn) | 15 |
0 | 2mm | 2mm | 0 (standardneedles) 13 (steel dpn) | 14 |
1 | 2.25mm | 2.25mm | 12 (steeldpn) | 13 |
1 | 2.5mm | 2.5mm | 1 (standardneedles) | N/A |
2 | 2.75mm | 2.75mm | 2 (standardneedles) 11 (steel dpn) | 12 |
2 | 3mm | 3mm | 3 (standardneedles) 10 (steel dpn) | 11 |
3 | 3.25mm | 3.5mm | 10 | |
4 | 3.5mm | 3.5mm | 4 (standardneedles) 9 (steel dpn) | N/A |
5 | 3.75mm | 3.75mm | 5 (standardneedles) 8 (steel dpn) | 9 |
6 | 4mm | 4mm | ||
N/A | 4.25mm | 4.25mm | 6 (standardneedles) | N/A |
7 | 4.5mm | 4.5mm | 7 | |
N/A | 4.75mm | 4.75mm | 7 (standardneedles) | N/A |
8 | 5mm | 5mm | 8 (standardneedles) | 6 |
N/A | 5.25mm | 5.25mm | 9 (standardneedles) | N/A |
9 | 5.5mm | 5.5mm | 5 | |
N/A | 5.75mm | 5.75mm | 10 (standardneedles) | N/A |
10 | 6mm | 6mm | 4 | |
101/2 | 6.5mm | 6.5mm | 101/2 (standardneedles) | 3 |
7mm | 7mm | 2 | ||
7.5mm | 7.5mm | 1 | ||
11 | 8mm | 8mm | 0 | |
13 | 9mm | 9mm | 00 | |
15 | 10mm | 10mm | 000 | |
17 | 12.5mm | |||
18 | 14mm | |||
19 | 15.5mm | |||
35 | 19mm | |||
50 | 25mm |
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